Journal: Cell reports

Article Title: Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma

doi: 10.1016/j.celrep.2020.108678

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay , Millipore , Cat# 48-612MAG.

Techniques: Produced, Virus, shRNA, Control, Transduction, Selection, Microarray, Recombinant, Cell Culture, Bradford Protein Assay, Multiplex Assay, Activity Assay, Software

Journal: bioRxiv

Article Title: Surface-modified measles vaccines encoding oligomeric, fusion-stabilized SARS-CoV-2 spike glycoproteins bypass measles seropositivity, boosting neutralizing antibody responses to omicron and historical variants

doi: 10.1101/2022.12.16.520799

Figure Lengend Snippet: (A) Isotype analysis of anti-SARS-CoV-2 spike antibodies. Serum samples from IFNAR −/− -CD46Ge mice vaccinated once (day 21) or twice (day 42) with MR-CoV-S6p312 were analyzed by ELISA for IgG1 and IgG2a antibody binding to SARS-CoV-2. Serum from mice vaccinated twice with purified SARS-CoV-2 Spike adjuvanted with alum was used as a control for the Th2-biased humoral response. (B) Cytokine production from the splenocytes of vaccinated mice. Splenocytes isolated from vaccinated mice were stimulated as indicated in , and cytokine secretion in the supernatant was analyzed by multiplex cytokine analysis. Dots represent individual animals, and horizontal bars and error bars are the mean ± SD. IL-1b lower limit of detection (LLOD): 1.45 pg/mL; IL-12 LLoD: 1.68 pg/mL; TNF-a LLoD 3.48 pg/mL; IFN-γ LLoD 2.19 pg/mL; GM-CSF LLoD: 3.20 pg/mL;IL-6 LLoD: 5.52 pg/mL; IL-5 LLoD: 2.19 pg/mL; IL-2 LLoD 1.88 pg/mL;IL-4 LLoD: 1.37 pg/mL; IL-13 LLoD: 2.86 pg/mL. Statistical significance was determined using two-way ANOVA with Dunnett’s multiple comparison test (*, p<0.05; **, p<0.003; ***, p<0.0003; ****, p<0.0001).

Article Snippet: Supernatants were then analyzed for the expression of IFN-γ, IL-6, IL-18, GM-CSF, IL-1β, IL12p70, IL-13, IL-2, IL-4, TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Purification, Isolation, Multiplex Assay

Journal: Parasitology international

Article Title: Cytokine Profiles in Opisthorchis viverrini Stimulated Peripheral Blood Mononuclear Cells from Cholangiocarcinoma Patients

doi: 10.1016/j.parint.2016.10.009

Figure Lengend Snippet: Cytokine profile of OvES stimulated and unstimulated PBMCs in uninfected control (A) and O. viverrini infected CCA patients (B). Levels of 11 cytokines were measured by FlowCytomix assay and flow cytometry in the PBMCs of individual from normal control (n=21) and CCA patients (n=61). The statistical significance was analyzed using Wilcoxon Matched-paired Signed rank test. *p<0.05.

Article Snippet: The secreted cytokines were quantified using commercial FlowCytomix bead-based multiplexing assays kit, human Th1/Th2 11 plex kit (BMS810FF, ebioscience) according to the manufacturer’s instruction and then assessed by flow cytometry (Beckman-Coulter).

Techniques: Infection, Flow Cytometry

Journal: Parasitology international

Article Title: Cytokine Profiles in Opisthorchis viverrini Stimulated Peripheral Blood Mononuclear Cells from Cholangiocarcinoma Patients

doi: 10.1016/j.parint.2016.10.009

Figure Lengend Snippet: Comparison of net cytokine production between PBMCs from CCA patients and control group. Levels of 11 cytokines were quantified by FlowCytomix assay and flow cytometry in the PBMCs of individual from normal control (n=21) and CCA patients (n=61). The statistical significance was analyzed using a Mann-Whitney U test. *p<0.05. The net cytokine production was calculated by cytokine level of stimulated with OvES minus cytokine level of unstimulated.

Article Snippet: The secreted cytokines were quantified using commercial FlowCytomix bead-based multiplexing assays kit, human Th1/Th2 11 plex kit (BMS810FF, ebioscience) according to the manufacturer’s instruction and then assessed by flow cytometry (Beckman-Coulter).

Techniques: Flow Cytometry, MANN-WHITNEY